In response to Kathy's question,
The recipe I have used is as follows:
7-AAD staining solution (25ug/mL in PBS with .1% sodium azide). Dissolve
2.5 mg of 7-AAD in to 100 mL of PBS with .1% sodium azide.
Store at 2 to 8 degrees C for up to a month.
Hope this helps,
Davin Jutila
DAVIN JUTILA, DIRECTOR SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY-1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU