Reply to Stephen Couzens' question
Hi Stephen
You might consider switching around fluorochromes as you suggest. I have=
used PerCP-CD45 with PE-CD34 and various FITC antibodies to look at CD34+=
subpopulations, including FITC-CD38, with good results. The CD45 and 34
were from BD, and the FITC-CD38 was from Immunotech.
Robin Barclay
SNBTS, Edinburgh