Sorting Question
Vincent Falco (vincent.falco@es.nemc.org)
Thu, 19 Jun 97 15:20:18 -24000
My problem is this:During some sorts I get ,what I will call a spurious extra
drop. To explain more,there are times I sort to polycarb filters.When all is
well I get a single spot which contains the desired cell population.When all
is supposed not well I get my desired single spot with cells and just about a
quarter of inch from the desired spot I observe a spurious spot,which does
not contain the desired cells.I maintain the nozzle on a strict PM cleaning
schedule and watch for clogs etc.during times of sorting.The drive frequency
remains constant during a sort and is generally consistent day to day.The
amplitude level requires adjustment during a sort run.Should I be suspect
that the amplitude level is giving the problem? Can anyone suggest other
potential problem areas.Can anyone give rules/guidelines they follow when
establishing drop drive frequency and amplitude level?Thanks.