I have been looking into the economics of our flow setup and discovered =
that I can buy PECy5 anti CD45 for much cheaper than I currently buy =
PerCP anti CD45. I proceeded to make some three color CD3/4/45 and =
3/4/45 PBL's to do some compensation with and found that I got into real =
trouble :(
Did something drastic go wrong with the labeling of the PBL's ??, or is =
there something I need to know about PE-Cy5 on a Calibur (ie: has anyone =
else done this easily/with difficulty ??)
One problem is that I have very little quality information on the =
Calibur - the manual seems to make no mention of the of exactly which =
mirrors/filters the Calibur uses ... does it use a 650LP filter on the =
FL3 detector the same as a FacsScan does ?? (if that's the case then I'm =
only just picking up the *tail* of the PE-Cy5 emission)
Peter Chapple
Melbourne AUSTRALIA