Identification of Eosinophils in Flow Cytometry -Reply

ANDRZEJ DEPTALA (ANDRZEJ_DEPTALA@nymc.edu)
Tue, 17 Jun 1997 19:08:10 -0500

You can use simultaneous detection: FLS/SS and CD45/CD14 MoAb
staining. The CD45-FITC/CD14PE bivariate distribution shows CD45-dull
cells, CD45-dim cells, CD45-bright cells and CD14+ cells. The FLS
versus SS distribution of CD45-bright cells shows eosinophils, which
are the most CD45-bright and CD14- among the granular cells.
Andrzej Deptala
The Cancer Research Institute, NYMC, Valhalla, USA