I am in need of a method to block Fc receptors on human cells. We have
antibodies (whole molecule) against a ligand on the surface of certain cells
that we want to use to block binding of those cells to their target - a
receptor on a human macrophage cell line. However, we need to prevent
binding to the Fc receptors on the macrophages via the Fc portion of our
antibodies. We prefer not to have to make F(ab')2 fragments of all of the
antibodies we will be testing.
I know that anti-CD16/32 monoclonals are available to block Fc receptors in
the mouse, but as far as I have been able to find out, the anti-human CD16
and CD32 monoclonals are not efficient as blocking agents.
If you have an efficient method of blocking human Fc receptors, please let
me know. And, thanks in advance.