Re: adherant cells
Mikael Vestberg (mikael.vestberg@inflam.lu.se)
Fri, 13 Jun 1997 13:05:41 +0200
>Could anyone suggest a method of removing adherant cell lines from culture
>vessels which does not remove surface adhesion molecules? At present i am
>studying surface cell adhesion molecules expression on human umbilical vein
>endothelial cells using flow cytometry.
>i would appreciate any suggestions.
>thanks
>Morgan
Try cell dissociation medium from Sigma. It works fine on endothelial cells
and is supposed to be protease free
Mikael