Green HeNe Laser - TexasRed/Cy3
O. Joseph Trask, Jr. (cytometry.osu.edu@postbox.acs.ohio-state.edu)
Tue, 10 Jun 1997 10:36:22 -0400 (EDT)
Greetings -
Is anyone out there using a Green NeHe Laser (~543nm) to excite Texas Red or
Cy3 for any type of immunophenotyping analysis, if so, how much output
power? Melles Griot has a 2mW Green NeHe Laser (543nm), but is 2mW output
power enough for immunofluorescence? I know 50mW from the 568nm line on a
Krypton Laser works well. All comments will be appreciated.
Regards,
O. Joseph Trask, Jr.
The Ohio State University
Comprehensive Cancer Center
Analytical Cytometry Laboratory
Tel: 614/292-FLOW(3569)
Fax: 614/292-7335
E-Mail: cytometry@osu.edu