Kevin G. Waddick, Ph.D.
Staff Scientist
Hughes Institute
2685 Patton Road
Roseville, Minnesota 55113
(612) 628-0598
(612) 628-9891 Fax
On Mon, 9 Jun 1997, Leanne Sammels wrote:
>
> Hello,
>
> I'm not sure that this is the right place to post this question
> but here goes. We have used in situ hybs to localise Murray
> Valley encephalitis virus RNA in the lymph nodes of mice
> infected IP with MVE. Only a small population of cells
> within the lymph node are positive for viral RNA and the
> morphology suggests it may be the follicular dendritic
> cells. I am interested in using flow cytometry to analyse
> infected lymph node cell suspensions(or maybe just
> peritoneal washouts?)to see if it is the dendritic cells
> which are replicating virus or if other cell populations
> are also involved in viral replication. Is this feasible?
> and if so can anyone recommend some publications which
> would be helpful. I'm sure it is quite obvious by now
> that I have had no cytometry experience whatsoever. Any
> advice would be appreciated.
> Kind regards,
>
> Lee Sammels
> Dr Leanne Sammels
> Department of Microbiology
> University of Western Australia
> Nedlands WA 6009
>
> Phone: 08 9346 4872
> Fax: 08 9346 2912
>