Re: pulse processing in cell cycle analysis

Larry Seamer (LSEAMER@COBRA.UNM.EDU)
Wed, 7 May 1997 16:43:23 +0000

In response to Fang-yao Hou, peak height measurements can under measure
total fluorescence when the particle being measured has a cross-sectional
diameter larger than the effective illumination spot of the laser. in
cell-cycle analysis this situation will result in a mean channel G2/G1 ratio
less than the theoretical 2.0 and often as low as 1.8. Therefore, pulse area
measurements are preferred when doing DNA staining.

On Tue, 6 May 1997, Fang-Yao Hou wrote:

>
> Dear FLOW experts:
>
> This is to ask your opinions about using FL-height, instead of FL-area in
> cell cycle analysis. I can see typical picture of G1, S and G2/M from FL-
> height histogram as long as I acquire data in linear mode. I am wondering
> if this is an acceptable way to do cell cycle analysis without purchasing
> the pulse processing module. The cytometer I am using is the BD Vantage.
> I appreciate your responses.
>
> Fang-Yao Hou
> FYHOU@macc.wisc.edu
>