Annexin V vs TUNEL

Diana Stone (dstone@vetmed.wsu.edu)
Fri, 2 May 1997 09:53:40 PST8PDT

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We have been using the TUNEL assay and dual staining for either surface
lymphocyte markers or internal staining for expression of a virus protein.
These cells (freshly isolated and short term cultured bovine peripheral blood
lymphocytes) are then analyzed by FACS. We have been getting very inconsistent
results....sometimes works, sometimes doesn't, or results make no sense.

Would we be better off switching to Annexin V for these dual staining studies
which require either concurrent external staining (B and T lymphocyte subsets)
or internal staining (viral protien) for the second molecule of interest?

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