does anyone have experience with a flow-based proliferation method that
doesn't require a lot of cells? i'm looking to measure cytokine dose
response from cell lines. the Brdu kits call for about a million cells
per test.
ideally, i want to titer the cytokine and culture the cells in 96 well
microtiter plates, and seed with 10E+03 or 10E+04 cells per well.
greatly appreciate if anyone has a method that can measure cell prolif
with so few cells or an alternative to 96well plates that can give
standard curve information.
woo