>I have recently been attempting Annexin V staining and so far this
>has been succesful. However I am worried about the amount of debris in the
>bottom left of FSC/SSC, should this be included in the final analysis
>or excluded?
>
I guess the answer depends on whether the events w/ low FS ansd SS are
apoptotic cells or debris or some other cell type [depending on the system].
For th e most part, the info you want is % apoptotic cells of a particular
population. So you need some method to determine whole cells [apoptotic or
live] from other events. If you can't achieve this with LS then maybe
adding a McAb or staining for DNA wil l be a required gating step.
Good luck,
Tom
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Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
4/23/97 11:28:52 AM
E-mail: thomasm@nshs.edu
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