pH measurements

Ken McDonald (kmcdonald@microimm.mcgill.ca)
Fri, 11 Apr 1997 11:35:37 -0500

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Chris Worth: "Transmembrane potential and Antibody staining..."
Previous message: J. Paul Robinson: "For Sale Elite Sorter"

Hi Flowers..I have a customer who is interested in measuring pH changes
in cells in relation to apoptosis.The paper he has shown me is from JBC
vol. 271, no 27 (pp 16260-16262) 1996.The paper refers to a method
called radiometric flow cytometry.We definitely don't want to do this
with our machine(Facs Vantage).I've heard there is a method using ratio
measurements(ie fl2/fl3) in which pH measurments can be made. Can anyone
give me any more details about this. I thank you in advance.
Ken McDonald

Next message: Chris Worth: "Transmembrane potential and Antibody staining..."
Previous message: J. Paul Robinson: "For Sale Elite Sorter"