Re: Yeast Cell Cycle Method

Larry Arnold (lwarma@med.unc.edu)
Tue, 8 Apr 1997 10:50:48 -0400 (EDT)

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At 01:21 PM 4/7/97 CST, you wrote:
>
>Does anyone have a good yeast cell cycle method? In particular, we need to
>eliminate a problem with the yeast clumping and try to get good single cell
>suspension. Have been using PI, but will try any method/dye that works well.
>Thank you, Martha
>Martha Kirby
>NIH, NHLBI, HB
>Bldg. 10, Rm 7C212
>Bethesda, MD 20892-1652
>kirbym@helix.nih.gov
>301-402-0277
>
>
Check out: Methods in Cell Biology : Flow Cytometry ,Second Edition Part B
, Chapter 27p 458. Several of our investigators have used this with success.

Larry Arnold
Director, Flow Cytometry Facility
Univ. of North Carolina at Chapel Hill

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