We have been trying to do intracellular staining for p25 protein (HIV
Ag) on HIV-infected cells. We have problems with cell loss after
PermeaFix fixation and permeabilization and with background and/or
non-specific staining (DAKO and/or Coulter p25 MoAb). Anybody out
there can help us?
Thanks in advance,
Regards to all of you.
Karolina Palucka