Platelet Activation via 3-color analysis

Michael F. Wolf (michael.wolf@medtronic.com)
Tue, 01 Apr 1997 13:58:13 +0100

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Looking for tips/feedback on doing a 3-color analysis of platelet
activation to compare degranulation (BD: CD62-PE) and gpIIb/IIIa
activation(BD: PAC-1-FITC) in heparinized human whole blood also
labeled with CD61 (BD: CD61-PerCP)as the pan-platelet marker.
Ultimately the purpose is to examine/compare platelet (and WBC)
activation events upon exposure of blood to common and surface-modified
versions of medical plastics used in cardiopulmonary bypass
applications. Any suggestions on optimal buffers and whether or not to
use fixatives? I'm somewhat new in the area but my limited experience
tells me that care is required in choice of the latter to avoid cell
lysis. M. Wolf

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