monoclonal antibody production
Meenakshi Roy (meena@macsun.stanford.edu)
Mon, 31 Mar 1997 21:30:39 -0800 (PST)
Dear flow-ers,
This is not a directly flow related question. I am planning to produce
monoclonal antibodies to certain cloned proteins.
My question is, what form of a protein is most effective for monoclonal
antibody production? I have been told that fusion proteins dont always
fold similarly to the native protein. So, monoclonal antibodies that
recognise the fusion protein, dont always recognise the native protein. On
the other hand, immunisation with transfectants can result in many
antibodies that are non-specific to the protein of interest.
Also, what are the relative merits of HA tagged fusionproetins, or Ig
chimera fusion proteins, or c-myc tagged fusion protiens for this purpose?
Any experiences, suggestions shall be highly appreciated,
Thanks in advance,
Dr. Meenakshi Roy
Stanford university