conjugation vs. sandwich -Reply
Antony Bakke (bakkea@ohsu.edu)
Thu, 27 Mar 1997 13:29:09 -0800
It is far easier to use a sandwich technique, if your primary antibody is
unconjugated. This can be successfully used even with 2 and 3 color staining.
Trying to conjugate an antibody is time consuming and expensive if you are
buying the unlabeled antibody. You need sufficient antibody to start - at least
100 ug and 1 mg would be better. After conjugating you must remove excess FITC,
unconjugated and over conjugated antibody.
If you need protocols for sandwich techniques, I'd be happy to send them.
Best regards,
Tony Bakke
bakkea@ohsu.edu