pH on FACScan -Reply

david_hedley@pmh.toronto.on.ca
Fri, 21 Mar 1997 18:18:57 -0500

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I have a lot of experience with flow pHi measurements, although not with
a FACScan. The filter set for FL2 and FL3 should be fine for use with
SNARF-1. In order to take the fluorescence ratio you will need software
such as WinList. The staining protocols for SNARF are generally
available. Let me know if you need any help.

David Hedley
Princess Margaret Hospital/Ontario Cancer Institute
Toronto, Canada

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