I'm looking for advice on the glycophoran A (GPA) assay used to look at M
and N allelic expression by flow. This is a new application for our
facility. We're currently using the BR6 assay described by Langlois et al
(Cytometry 11:513, 1990). However, we're having problems getting
consistent light scatter profiles and with the results in general. I think
part of the problem may be with the fixation.
Any suggestions or comments??? Thanks.
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Julie A. R. Pribyl
LeBien Lab/ U of MN Cancer Center
Lab phone: 612-626-4839
Flow Cytometry Phone: 612-624-7151
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