Re: Transit time

LEKAHN@monsanto.com
Fri, 7 Mar 1997 07:59:38 -0600

The device I know about is made by Cytek (510)657-0102. It is called
a Time Zero Module and I think it's around $5000. I have also seen
some papers in Cytometry over the years with more lab-made devices
that might work for you.

Larry Kahn
Searle Discovery Research

______________________________ Reply Separator _________________________________
Subject: Transit time
Author: owner-cyto-sendout@flowcyt.cyto.purdue.edu at INTERNET
Date: 3/6/97 6:33 PM

Dear Group,

An investigator wants to look (using flow) at Ca++ responses that occur
within seconds (as in 1 or 2 seconds) after the addition of a particular
agent to dissociated endothelial/epithelial cells. Can anyone tell us
the fastest transit time from sample uptake to laser intercept
with either a Coulter Elite or FACSVantage? I also seem to remember a
commercially available device that could be attached to (any?) flow
cytometer and that increased the speed of sample injection. Does anyone
know the particulars about this device - manufacturer, cost, and how well
it works? Can it can be attached to both the Coulter Elite and the
FACS Vantage?

Thanks for any info.

Ray

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Raymond B. Hester, Ph.D.
Flow Cytometry Lab
CSAB 357, Biotechnical Services
The University of South Alabama
Mobile, AL 36688-0002 To err is human -
Email: rhester@jaguar1.usouthal.edu but it feels divine.
Voice: