______________________________ Reply Separator _________________________________
Subject: Re: Sorting into Terasaki plates
Author: jim.houston@stjude.org at INTERNET
Date: 04/03/97 21:11
On Sat, 1 Mar 1997 ClaudeC81@AOL.COM wrote:
>
> I have been asked to test / set up our single cell deposition system for
> sorting single cells into Terasaki plates. Sounds like a challenge to me,
> but can it be done? FACStar Plus, ACDU, macrosort head. Isn't that sorted
> drop going going to make like a giant meteor on earth and blow all to well?
> Is the sorter accurate enough to spit a drop / cell into such a small well /
> volume in the first place? Answers to these and other questions would be
> much appreciated, as well as tips on how to do it... if it can be done!
> Thanks, Claude
>
I have done it on a Vantage with a TSO. It takes a bit of time to adjust
the well differences. Be patient. It is not if the sorter can do it, but
can the operator? Use 10-12 psi sample pressure.
Good luck,
Jim