Ethanol also is a most effective fixative agent (!) & as such will
fix any residual protein, et. al. on to the walls of your instrument's
tubing, flow cell, etc. very efficiently as well!
"Zip".
>>>--->
----------------------------------------------------------------------
> Date sent: Mon, 24 Feb 1997 11:44:40 +0000
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> From: Arnold Pizzey <a.pizzey@ucl.ac.uk>
> Subject: Clean machines
>
>
> Greetings all.
>
>
> I would question the efficacy of using 70% ETOH alone as a
> sterilization agent specifically in flow as well as generally in the
> laboratory. Although ETOH has some bactericidal activity against common
> water-borne contaminants such as the pseudomonads and the like I would
> imagine that most airborne contaminants that float into the sheath tank
> would be of the Gram-positive persuasion and as such quite resistant to the
> effect of ETOH.
> As for swabbing down external surfaces with 70% ETOH, I would regard this
> as an excellent strategy for mobilizing bacterial aerosol.
>
> On the few occasions when I have had to carry out sterile sorting, my
> strategy is as follows:
>
> Clean sort nozzle/flow cell /sheath tank with a proprietary detergent and
> rinse in sterile H2O.
> Wash the sort collection area down with a proprietary detergent
> Bypass the in-line sheath filter, Then flush system with following:
> 1)H2O
> 2)Surface active agent e.g. 0.1% DECON 90
> 3)Sterile H20
> 4)Sterile sheath fluid
> 5)Fit new sterile in-line sheath filter.
>
> Regards .
>
> Arnold.
> _____________________________________________________
> Arnold Pizzey
> Department of Haematology
> 98 Chenies Mews
> London WC1E 6HX
> E-mail a.pizzey@ucl.ac.uk.
> Voice (0)171 209 6234;
> fax (0)171 209 6222.
> _____________________________________________________
>