--------------------------------------
Date: 2/11/97 4:21 PM
To: cyto-inbox
From: Jim Zanghi
>Small beads staying in the instrument can be a problem. Triton X-100 at
>0.1% (the same concentration used to permeabilize cells) in water will
>remove most of them.
>
>Betsy Robertson
>>
>> Running ethanol
>> after the beads seems to help push them through more quickly.
>>
>> Tony Bakke
I was wondering about what types of solutions can be run through a flow
cytometer without causing damage or corrosion. Is pure ethanol okay? On
the advise of others, I have run 50% hot bleach to clear up clogs, but I
was very weary about doing this since bleach corrodes stainless steel.
I've always followed with a 5 minute water rinse, but this concentration of
bleach seems a bit extreme (we routinely use 10% bleach at room temp).
If 0.1% triton or 70% ethanol is as effective as 50% hot bleach, than this
seems to be the way to go. Any thoughts about this? What do
manufacturers recommend?
Jim
-- James A. Zanghi Dept. of Chemical Engineering Northwestern University Evanston, IL
------------------ RFC822 Header Follows ------------------ >From flowcyt.cyto.purdue.edu!owner-cyto-sendout Tue Feb 11 14:08:37 1997 remote from donews Received: from flowcyt.cyto.purdue.edu by donews.cts.com with smtp (Smail3.1.29.1 #5) id m0vuQNO-00005zC; Tue, 11 Feb 97 14:08 PST Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyto-sendout id MAA10161; Tue, 11 Feb 1997 12:30:01 -0500 Received: from merle.acns.nwu.edu by flowcyt.cyto.purdue.edu via ESMTP (940816.SGI.8.6.9/930416.SGI.AUTO) for <cytometry@flowcyt.cyto.purdue.edu> id LAA09604; Tue, 11 Feb 1997 11:18:55 -0500 Received: from [129.105.205.225] (barney.chem-eng.nwu.edu) by merle.acns.nwu.edu with SMTP (1.40.112.8/16.2) id AA138027530; Tue, 11 Feb 1997 10:12:11 -0600 Message-Id: <v01510103af264a551654@[129.105.205.225]> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 11 Feb 1997 10:19:08 -0600 To: cyto-inbox From: zanghi@merle.acns.nwu.edu (Jim Zanghi) Subject: Re: A clean machine -Reply