I was wondering if somebody has experience detecting fusionproteins with a
"flag" epitope, using a commercial available anti-flag antibody for
intracellular expression...
I haven't tried it, but would like to know if this could possibly work,
especially for somebody who want's to analyse cellcycle expression of a
transfected protein using a flag-fusion construct.
Any details on which mAb to use, minimal expression levels, staining
procedures would be very wellcome.
Thank you very much,
Matthias
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Matthias Haury Flowcytometry Dept Immunology Institut Pasteur
mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39
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