FITC staining for YEAST

Jihan Marjan (jmarjan@gpu.srv.ualberta.ca)
Thu, 6 Feb 1997 10:27:15 -0700 (MST)

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I got this address from Dorothy at Medical Sciences Building,
University of Alberta, Edmonton.
The project invoves using S.C. yeast with a mammalian construct
inserted. We would like to confirm the presence of the mammalian
construct within the yeast using a FACS scan and antibodies against
epitopes iwthin the mammalian construct. Is the procedure for this
protocol the same as with mammalian cells with the addition of a 45 second
sonication step at the end before running a FACS scan?
I appreciate any comments you may have with regards to this
question?
Yours sincerely,
Jihan Marjan Ph.D.

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