Acridine Orange is probably the fastest and the easiest method to
>evaluate apoptosis and/or cell cycle but ..... how can I be sure that it
>wouldn't contaminate our flow-cytometer?
On our Elite, we routinely change the sample tubing between applications
where a dye is known to remain in the tubing. You'll have to check how
feasible this is for your machine, but is a simple way to avoid
contamination of subsequent samples.
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Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
2/5/97 1:12:01 PM
E-mail: thomasm@nshs.edu
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