What you can use is FACS-LYSING-SOLUTION from Becton and Dickinson,
and vortex very well and incubate for 5 min at roomtemperature.
succes Cor Jacobs
C. Jacobs
Department of Nephrology
University Hospital Nijmegen
Nijmegen, the Netherlands
E-mail: c.jacobs@nefro.azn.nl