I was wondering whether anyone has tried determining the copy number
of a gene inserted into the nucleus by transformation. We are trying
to determine the copy number of a gene which we have
transformed into tomato plants. This gene interferes with PCNA
resulting in endopolyploidy. I would therefore like to see if I
could correlate copy number with ploidy level, and
what is the expression level of PCNA in these cells.
Any suggestions would be most welcome,
Thanks
Iona O'Brien.