Bone marrow volume

Kevin G Waddick (waddi002@maroon.tc.umn.edu)
Fri, 17 Jan 1997 18:15:13 -0600 (CST)

We are the reference lab to which all BM samples from suspected and
confirmed pediatric ALL patients enrolled in Children's Cancer Group
(CCG) clinical trials are sent. In addition, we monitor the status of
patients being treated in FDA trials with immunotoxins originating from
our program. In other words, we deal with large numbers of BM samples
from patients. What we ask for, and what has worked well, is 5 cc of BM
aspirate. From that volume, we do flow cytometric immunophenotyping and
sorting for enumeration of clonogenic progenitor cells in colony assays.

Kevin G. Waddick, Ph.D.
Asst. Professor
University of Minnesota
Biotherapy Institute
2625 Patton Road
Roseville, Minnesota 55113
(612) 627-1920 ext. 131 Office
(612) 627-1928 Fax

On Fri, 17 Jan 1997, Maryalice Stetler-Stevenson wrote:

>
> Question for Clinical Flow People:
>
>
> What volume of bone marrow do you aspirate for flow. If you aspirate too
> much you get blood, not bone marrow. However, you want as much as possible
> from a single site. I had thought 1mL per site was maximum but was told
> today that 3 mL gives a good yield. Please share you specifications and I
> will summarize for those interested.
>
>
> Maryalice
>
> Maryalice Stetler-Stevenson
> Director Flow Cytometry Unit
> Laboratory of Pathology, NCI, NIH
>
>