Apoptosis detection with Annexin V and DiOC6

Christian =?iso-8859-1?Q?St=F6ckigt?= (stoeckig@uni-duesseldorf.de)
Fri, 17 Jan 1997 22:19:40 +0100

Hi Flow- ers!

We are trying to detect induced apoptosis with Annexin V and DiOC6 staining
followed by FACScan analysis. Until now, our results are not satisfying-
Staining with rhodamine works well.
Does anyone know a working protocol (apart from any datasheet or catalog
information!) and/or has experience with these staining methods? Please
reply directly to my Email

Thanks in advance

Christian Stoeckigt

Immunology Laboratory
Institute for Hemostasis and Transfusion Medicine
Heinrich- Heine University Medical Center
Duesseldorf, Germany

stoeckig@uni-duesseldorf.de