Re: Reed-Sternberg Cells
Anna Porwit (anpo@mb.ks.se)
Fri, 17 Jan 1997 10:35:50 +0100
>
>Hello,
>
>Are Reed-Sternberg/CD30 (ki-1) cells generally found by FCM methodologies?
>Are there any tripple staining techniques to specifically select for these
>cells? Maybe CD30 FITC with CD5 PE and CD19 CY5-PE ? What are the light
>scatter properties Reed-Sternberg cells? We have not had the opportunity to
>correlate FCM-CD30 with histological staining. ANY comments would be
>appreciated. The literature suggests some expression of CD30 in some
>non-Hodgkins lymphomas.
>
>----------------------------------------------
>Bill Baughman
>Americal Medical Laboratories, Inc., FCM
>E-mail: fcm@amlimmun.dgsys.com
>----------------------------------------------
>
>Hi,
There are cases of higly malignant T cell NHL and even very rare cases of
large cell B-cell NHL (which are not KI-1 NHL by morphology and other
markers) that are CD30 positive. Moreover occasional T cells in HD lymph
nodes can be CD30+.
R-S cells are difficult to get in suspension and FCM methods are of very
limited use in diagnosis. We usually do immunostaining on paraffin/frozen
sections.
Best wishes
Anna Porwit-MacDonald
Haematopathology, Dept. of Pathology,
Karolinska Hospital, Stockholm, Sweden
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