We have a project in which we would like to study DNA ploidy plus protein
expression of two nuclear antigens. Our intrumentation is limited to a
FACSCAN. I assume that we will start by using FITC and propidium idodide.
But what, if any, is your recommendation for a third color? Is this
feasible with the limitation of our instrument?
Thanks.
Howard Ratech, MD
Albert Einstein College of Medicine/
Montefiore Medical Center
Bronx, New York