Ubiquitin

Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Mon, 13 Jan 1997 23:00:25 -0500

We are trying to stain PFA fixed RBL (rat basophilic leukemia cells)
for intracellular ubiquitin. I have tried the Ubi-1 clone from Zymed
without success (although it works well for Western).

There are a number of different clones available, however I have been
told by one company that their clones definitely do not work in rat
cells for flow. Rather than randomly try other clones, I'd appreciate
finding out if anyone has experience staining for ubiquitin in ANY
species by flow?
Any suggestions? Thanks.

Calman