A) Fluorescence pulse area (FPA) and fluorescence pulse width (FPW) are used simultaneously as parameters. Groups corresponding to six pairs of chromosomes (higher values of FPW, similar values of FPA) are resolved. Chromatid doublets of different chromosomes are shown also.

B) Contour plot of MI/DAPI stained field bean chromosomes. The chromosomes are stained with two fluorochromes, 4',6-diamidino- 2-phenylindole (DAPI) at final concentration of 1.5 µM and mithramycin A (MI) at final concentration of 20 µM for at least 30 min. MgSO4 is added to chromosome suspension at final concentration of 10 mM prior to staining.