BMS 632

Flow Cytometry


credit hours: 3
when offered: Spring Semester

Instructor
	J. P. Robinson, Basic Medical Sciences

Prerequisites: None

Description
A thorough excursion into the theory of flow cytometry.  This course seeks
 to understand what the technology is, how it operates, and where the 
applications exist.  This course will cover optical systems, including 
filters, light excitation and emission, fluorescent dyes, and fluorescent
 molecules, electronics including detection systems, signal processing, 
data analysis, and computer applications.  A discussion of lasers and other
 light sources as well as quality control.  Finally, an overview of the
 applications areas in medical science, biological research, and other 
possible areas of use.
 
Text
Introduction to Flow Cytometry, J. Watson, Cambridge Press, 1991.
Flow Cytometry: First Principles, Alice Longobardi Givan, Wiley-Liss, 1992.
Flow Cytometry: A Practical Approach, M. G. Omerod, IRL Press, 1990.
Methods in Cell Biology: vols 40, 41, Darzynkiewicz, Robinson & Crissman, Academic Press, 1994.
Flow Cytometry, Advanced Research and Clinical Applications, A. Yen, CRC Press.

outline
Week	Topic
1.	Introduction to the course
2.	The history of flow cytometry
3.	How a flow cytometer works - Optics
4.	How a flow cytometer works - Light Sources
5.	Midterm quiz
6.	How a flow cytometer works - Electronics
7.	How a flow cytometer works - Fluidics
8.	How a flow cytometer works - Data Collection
9.	Reviews
10.	Data analysis - Section 1
11.	Data analysis - Section 2
12.	Presentation of Student Seminar
13.	Course evaluations and review

For further information regarding the Biomedical Engineering Program at Purdue University contact the Biomedical Engineering Graduate Office at (317) 494-5730 bmeprogram@ecn.purdue.edu