ChE 697E

Optical Engineering for Bio-Sensing


3 credit hours
Spring semester; alternate years

instructor
	E. M. Sevick-Muraca

prerequisites
A strong background in transport phenomena and an interest in electro-optical
 instrumentation. 

description
With the advent of economical laser diode and detector technologies, the
 use of optical devices in industrial and medical applications continues 
to increase.  Yet there is little formal introduction to the physics and 
instrumentation behind diagnostic and analytical optics for use in process
 monitoring and diagnostics in the biotechnology and medical industries. 
 While numerous vendors package and sell optical diagnostic devices in the
 medical and chemical process monitoring industries, engineers are often
 ill-equipped to judge the practicality and the applicability to real
 systems of interest.  This is increasingly the case in the chemical 
and pharmaceutical industries.  In this course, students will investigate
 the physics behind light interaction with inert and optically active 
random media and describe mathematics of light propagation these systems.
  Practical application of various instrumental approaches will be evaluated
 for the pharmaceutical, chemical process, medical, and biodiagnostic 
industries.  The course will be divided into two parts:  in the first
 part, students will concentrate on biomedical tissue optics and in the
 second, upon process monitoring, analeptics, and photochemistry will be
 reviewed as the pertain to optical monitoring.  Fluorescence, luminescence,
 Raman, absorption, and scattering techniques will be discussed as they
 pertain to traditional (non-scattering) samples and the random systems
 which constitute the majority of industrial optics applications.

text
Notes

outline	
	Topic	Number of Lectures
Review: particle and wave description of light	1
Absorption spectroscopy in biological systems	1
Vibrational spectroscopy in biological systems	1
Fluorescence spectroscopy in microscopy applications	1
Dyes, reporters in tissues, cultures, and microscopy 	2
Phosphorescence spectroscopy in biological systems	1
Instrumentation	2
Single-photon counting for fluorescent lifetime and yield	1
Frequency-domain measurements for fluorescent lifetime and yield	1
Lifetime and yield computations for bio-chemical sensing	1
Theories of light scattering by single particles	1
Radiative transfer equation	3
Monte Carlo simulation of light propagation	1
Diffusion approximation to radiative equation	1
Boundary conditions for light propagation in random media	1
Solutions to description of light propagation in time and frequency domains	1
CW, time, and frequency domains	1
Diffusing Wave Spectroscopy 	2
Mathematical description of fluorescent/phosphorescent re-emission 	1
within random media
Inverse problems in optical engineering	1
Chemometrics for optical process monitoring 	2
Determination of particle size	2
Particle sizing from Turbidity, specular reflection, DWS, time-dependent light 	1
propagation
Bio-sensing in random media using phosphorescence measurements	2
Optical Imaging in Random Media 	3
Advanced instrumentation for imaging	1
Solutions to the inverse imaging problem 	4
Project presentations	4

For further information regarding the Biomedical Engineering Program at Purdue University contact the Biomedical Engineering Graduate Office at (317) 494-5730 bmeprogram@ecn.purdue.edu