SpheroTECHNICAL NOTES

The following SpheroTECHNICAL NOTES are available upon request:

STN-1: Particles Coating Procedures

STN-2: Determination of Antibody binding to particles

STN-3: Binding Capacity of Avidin Magnetic Particles

STN-4: Binding Capacity of Gt-anti-Ms-IgG Magnetic Particles

STN-5: Binding Capacity of Streptavidin Magnetic Particles

STN-6: Binding Capacity of Biotin Magnetic Particles

STN-7: Separation of Mononuclear Cells from Peripheral Blood using SPHERO
Gt-anti-Ms-IgG Magnetic Particles

STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO
Calibration Particles

STN-9: Measuring MESF with Flow Cytometer Using SPHERO Rainbow
Calibration Particles

CHARACTERISTICS OF POLYSTYRENE PARTICLES

Density: 1.05

Refractive Index: 1.59

Composition: Linear polystyrene

Shape: Uniform microspheres

Porosity: Nonporous

Compatibility with organic solvent: Inert to alcohol and DMSO but soluble in DMF, acetone, acetonitrile, xylene, chloroform and methylene chloride.

Functional groups: Located on the surface with alkyl linker arms.

Functional group contents:

0.8 um Carboxyl particles: ~ 50 ueq/g solid

0.8 um Amino particles: ~ 15-20 ueq/g solid

AVIDIN POLYSTYRENE PARTICLES (0.8 um):

Covalently coated with egg white avidin

Avidin Contents : ~ 14 ug/mg solid; ~ 0.212 nmole/mg solid

Binding capacity to Biotin-Fluorescein: ~ 0.46 nmole/mg solid

ANTIBODY COATED POLYSTYRENE PARTICLES (0.8 um):

Antibody contents: ~ 14 ug/mg solid; ~ 0.2 ug/sq cm; ~1.5x104 IgG/particle

Binding capacity to IgG-FITC: ~ 4 ug/mg

VOLUME, NUMBER AND SURFACE AREA VS. PARTICLE SIZE

Volume of particle: V = (3.14/6) x D3 x 10e-12 cm3/particle

Number of particles: N = (6W/3.14PD3) x 10e12 particles

Surface area of particles: A = (6W/PD) x 10e4 cm2

Where
W = Weight of polymer in gram
P = Density of polymer (polystyrene = 1.05)
D = Diameter of particles in micrometer

Total surface area of 1 ml of 5% w/v (50 mg) particle:

A = 2857/D cm2

Total surface area of 20 ml of 0.25% w/v (50 mg) particles:

A = 2.857/D cm2

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For 1 ml of 1% w/v (10 mg) particles

DIAMETER(um)--SURFACE AREA(cm2)------#(x10e9)

0.05--------------------11428--------------------------145513
0.10----------------------5714---------------------------18189
0.50----------------------1143---------------------------145.51
0.80-----------------------714----------------------------35.53
1.0-------------------------571--------------------------18.19
1.5-------------------------381----------------------------5.389
2.0-------------------------286----------------------------2.274
2.5-------------------------229----------------------------1.164
3.0-------------------------190----------------------------0.6737
3.5-------------------------163----------------------------0.4242
4.0-------------------------143----------------------------0.2842
4.5-------------------------127----------------------------0.1996
5.0-------------------------114----------------------------0.1455
5.5-------------------------104----------------------------0.1093
6.0---------------------------95----------------------------0.0842
6.5---------------------------88----------------------------0.06625
7.0---------------------------81----------------------------0.0530
8.0-----------------------------------------------------------0.03552
10.0--------------------------------------------------------0.01819
12.0--------------------------------------------------------0.01052
15.0--------------------------------------------------------0.00539

SPHEROTECH IS THE BEST SOURCE FOR UNIFORM MICROPARTICLES

Last modified on Thursday, March 27, 1997