1. Mechanics of a flow cell

2. How foward angle light scatter is collected

3. How 90 degree light scatter is collected

4. Fluorescence detection in the flow cell

5. Fluoresecence activated cell sorting in the flowcytometer

6. Optical filters - an example of a dichroic filter

7. Mechanism of a band pass filter

8. Examples of long and short pass filters

9. Diagramatic representation of the optic layout of a flow cytometer

10. Common excitation and emission spectra of various fluorescent probes

11. Excitation and emission spectra of propidium iodide

12. Excitation and emission spectra of FITC

13. Excitation and emission spectra of PE

14. Excitation and emission spectra of APC

15. Diagram of typical research cytometer (Coulter 753)

16. Diagram of typical clinical cytometer

17. Diagram of microscope and its optics

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu