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>
> We are interested in sorting viable human monocytes (CD14+) to be used in
> functional cytokine production assays. We have done the experiment once
> with poor monocyte viability. The monocytes were sorted, placed into
> culture for 24 hours and then stimulated with LPS and monensin. The
> combination of these events killed the monocytes - any suggestions? Thanks
> in advance for any suggestions. Stacie Anderson
>
> Stacie Anderson
> Laboratory of Gene Transfer, Hematopoeisis Section
> NCHGR, NIH
> Room 2C12
> 49 Convent Drive
> Bethesda, MD 20892-4442
>
>
>
Try sorting into 5 ml polypropelene tubes with 1/2 ml HI/FCS in the tube.
Keep the collected cells cold!!!
You can also try sorting into a petri dish with HI/FCS.
Jim
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
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PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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