Re: Monocyte sorting

Jim Houston (
Mon, 30 Jun 1997 14:26:22 -0500 (CDT)

On Fri, 27 Jun 1997, Stacie Anderson wrote:

> We are interested in sorting viable human monocytes (CD14+) to be used in
> functional cytokine production assays. We have done the experiment once
> with poor monocyte viability. The monocytes were sorted, placed into
> culture for 24 hours and then stimulated with LPS and monensin. The
> combination of these events killed the monocytes - any suggestions? Thanks
> in advance for any suggestions. Stacie Anderson
> Stacie Anderson
> Laboratory of Gene Transfer, Hematopoeisis Section
> Room 2C12
> 49 Convent Drive
> Bethesda, MD 20892-4442

Try sorting into 5 ml polypropelene tubes with 1/2 ml HI/FCS in the tube.
Keep the collected cells cold!!!

You can also try sorting into a petri dish with HI/FCS.


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