Re: Sorting of Sperm?

Howard Shapiro (
Wed, 2 Jul 1997 22:59:45 -0400 (EDT)

>I have been asked to gather all of the information I can on sperm sorting.
>Specifically, I need information on the determinants used to separate X and Y
>bearing sperm. Thanks, in advance, for any information.
>Kathy McKinnon

The expert on sperm sorting is Larry Johnson at the U. S. Department of
Agriculture in Beltsville, which is a short drive away from your facility.
He and most other people who have successfully separated X and Y sperm have
relied on detection of differences in DNA content, using modified commercial
flow cytometers and staining cells with Hoechst 33342. Surface markers
evaluated to date have apparently not been as effective.


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