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We have been using -342 to identify bovine sperm, then sorting into
microwell plates for PCR. No problems seen with the -342 complicating the
PCR wizardry.
Here are a few references from this work.
Lewin, H. A., K. Schmitt, R. Hubert, M. J. T. van Eijk and N. Arnheim.
1992. Close linkage between bovine prolactin and BoLA-DRB3 genes: genetic
mapping in cattle by single sperm typing. Genomics 13:44-48.
Van Eijk, M. J. T., I. Russ and H. A. Lewin. 1993. Order of bovine DRB3,
DYA and PRL determined by sperm typing. Mammalian Genome 4:113-118.
Park, C., I Russ, Y. Da, and H. A. Lewin. 1995. Genetic mapping of F13A to
BTA23 by sperm typing: difference in recombination rate between bulls in
the DYA-PRL interval. Genomics 27: 113-118.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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