Sperm sorting

Gary Durack (durack@ux1.cso.uiuc.edu)
Wed, 15 Jan 1997 10:53:06 -0600 (CST)


We have been using -342 to identify bovine sperm, then sorting into
microwell plates for PCR. No problems seen with the -342 complicating the
PCR wizardry.

Here are a few references from this work.

Lewin, H. A., K. Schmitt, R. Hubert, M. J. T. van Eijk and N. Arnheim.
1992. Close linkage between bovine prolactin and BoLA-DRB3 genes: genetic
mapping in cattle by single sperm typing. Genomics 13:44-48.

Van Eijk, M. J. T., I. Russ and H. A. Lewin. 1993. Order of bovine DRB3,
DYA and PRL determined by sperm typing. Mammalian Genome 4:113-118.

Park, C., I Russ, Y. Da, and H. A. Lewin. 1995. Genetic mapping of F13A to
BTA23 by sperm typing: difference in recombination rate between bulls in
the DYA-PRL interval. Genomics 27: 113-118.

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