Enoc Hollemweguer (
27 Nov 1996 07:51:10 -0800

There are several articles on PNH, and a couple of recent papers come to mind.
You might want to read through these and find them helpful.

1. Rosse, W. F., and R. E Ware. 1995. the molecular basis of Paroxysmal
Nocturnal Hemoglobinuria. Blood. 86: 3277.

2. Hall, S. E., and W. F. Rosse. 1996. The use of monoclonal antibodies and flow
cytometry in the diagnosis of Paroxysmal Nocturnal Hemoglobinuria. Blood. 87:

You could also send a fax to (619)812-8888 and request information on reagents.

Good Luck

Date: 11/26/96 8:52 PM
To: cyto-inbox
From: Walter Sharp

Second query today !

I would like to ask for a concensus opinion on the use of antibodies to GPI
linked antigens such as 55, 59, 14, 16 etc in the diagnosis of PNH and the early
detection of conversion of Aplastic anaemia to PNH.

We routinely use 55 (sometimes - see earlier mail re: Heat stability) & 59 in
parallel to the old Sucrose lysis and "Ham's" test.

Two questions -

1) Does the "panel" think that 55 & 59 are sufficient or should others like 14
and 16 be used also, as is suggested in various papers ?

2) Is it really necessary to use stone age techniques such as Sucrose lysis and
acidified serum tests when the "cutting edge" technology is available ?

Thanks again

Wal Sharp

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Date: 26 Nov 96 08:47:37 EST
From: Walter Sharp <102675.320@CompuServe.COM>
To: cyto-inbox
Subject: PNH
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