phytoplankton sorting

John Mahoney (
25 Jan 1997 13:52:43 -0500

Response to Sheila Agawin,

I am a relative novice with our FACS Vantage but have sucessfully
sorted several phytoplankton species without difficulty. These
included flagellates Olisthodiscus luteus, Prorocentrum triestinum,
Gyrodinium aureolum and a picoplankter, Aureococcus anophagefferens.
All survived sorts except for A. anophagefferens single cell ACDU
isolation attempts. I usually use glass 100mm test tube with slip on
caps for collection but plastic tubes have worked also. Tubes are
filled about three quarters with media and positioned high in the tube
holder. Cooling is not a problem because the unit is in an air
conditioned room and I use several freezer packs in the collection
compartment in lieu of setting up refrigeration. Dave Small,
Becton-Dickinson, recommends varying sheath pressure. Good Luck!

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