Re: Methods for sorting viable X and Y sperm

Gretchen Lawler (
Wed, 18 Jun 1997 08:50:49

There is an immunomagnetic method. See Jones, P.P., Peter, A.T., and
Robinson, J.P.: An immunomagnetic method to separate X and Y
chromosome bearing mammalian spermatozoa.
Proc.Int.Cong.Anim.Reprod.Artif.Insem. 1:484-486, 1992.

> Using an orienting flow nozzle and staining for DNA content using
> Hoechst 33342 is the only reliable way to sort viable sexed sperm.
> While the idea of using oligos makes sense, the problem is that you
> have to kill the sperm to get the oligos into the DNA. No one to my
> knowledge has been able to come up with any specific surface markers
> that could be used to distinguish X from Y sperm without having to
> fix the sperm.
> Mike Fox
> On Mon, 16 Jun 97 11:56:27 -0600 wrote:
> >
> > What kind of methods are being used to sort viable X and sperm in particular
> > bull sperm. A graduate student has brought me some older literature
> > describing sorting on differences in DNA content using specialized adaptors
> > to maintain consistent orientation of the sperm as they pass through the flow
> > cell. Is it possible to use FITC oligos to specific regions of the Y
> > chromosome to accomplish this? I have sent the graduate student back to search
> > out more on this possibility, but am wondering if anyone out there is already
> > doing this or has found it to be impossible, or is using another even more
> > elegant method? The graduate student is quite keen and quite green so I am
> > trying to make sure he isn't biting off more than he can chew.
> >
> >
> > regards
> >
> >
> > BJC - Brian
> Mike Fox
> Chairman, Cell and Molecular Biology Graduate Program
> Dept. of Radiological Health Sciences
> Colorado State University Ph: (970)491-7618
> Ft. Collins, CO 80523 USA Fax:(970)491-0623
> ====================================

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web , EMAIL