Re: adherant cells

Mikael Vestberg (
Fri, 13 Jun 1997 13:05:41 +0200

>Could anyone suggest a method of removing adherant cell lines from culture
>vessels which does not remove surface adhesion molecules? At present i am
>studying surface cell adhesion molecules expression on human umbilical vein
>endothelial cells using flow cytometry.
>i would appreciate any suggestions.

Try cell dissociation medium from Sigma. It works fine on endothelial cells
and is supposed to be protease free


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web , EMAIL