Re: adherant cells

Deborah Berglund (
Fri, 12 Jun 1992 12:33:49 -0600 (MDT)

We do this all the time. If you can, try using a CaMg-free incubation;
we use Tyrode's. 10-15 min incubation in incubator, change it twice, then
rinse the cells off. If they don't come off, try a very short (10-20
sec) exposure to
trypsin or trypsin/EDTA, then rinse off with culture medium. Get back to
me if this doesn't work.

Deb Berglund
Montana State University
On Thu, 12 Jun 1997 wrote:

> Could anyone suggest a method of removing adherant cell lines from culture
> vessels which does not remove surface adhesion molecules? At present i am
> studying surface cell adhesion molecules expression on human umbilical vein
> endothelial cells using flow cytometry.
> i would appreciate any suggestions.
> thanks
> Morgan

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