Re: adherant cells

Deborah Berglund (umbbd@gemini.oscs.montana.edu)
Fri, 12 Jun 1992 12:33:49 -0600 (MDT)

We do this all the time. If you can, try using a CaMg-free incubation;
we use Tyrode's. 10-15 min incubation in incubator, change it twice, then
rinse the cells off. If they don't come off, try a very short (10-20
sec) exposure to
trypsin or trypsin/EDTA, then rinse off with culture medium. Get back to
me if this doesn't work.

Deb Berglund
Montana State University
On Thu, 12 Jun 1997 ans033@abdn.ac.uk wrote:

>
> Could anyone suggest a method of removing adherant cell lines from culture
> vessels which does not remove surface adhesion molecules? At present i am
> studying surface cell adhesion molecules expression on human umbilical vein
> endothelial cells using flow cytometry.
> i would appreciate any suggestions.
> thanks
> Morgan
>


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu