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Is it possible to use FITC oligos to specific regions of the Y
>>chromosome to accomplish this? I have sent the graduate student back to
>>search
>>out more on this possibility, but am wondering if anyone out there is already
>>doing this or has found it to be impossible, or is using another even more
>>elegant method?
>
>I doubt that you could get the oligos to hybridize while retaining
>viability. I keep hearing that there are X- and/or Y-specific surface
>antigens, which would be ideal. Larry Johnson of the U. S. Department of
>Agriculture probably knows more about sex selection by sperm sorting than
>anybody else. Some references from a few years back are:
>
>Cran DG, Johnson LA, Miller NG, Cochrane D, Polge C: Production of bovine
>calves following separation of X- and Y-chromosome bearing sperm and in
>vitro fertilisation. Vet Rec 132:40-1, 1993
>
>Hendriksen PJ, Tieman M, Van der Lende T, Johnson LA: Binding of anti-H-Y
>monoclonal antibodies to separated X and Y chromosome-bearing porcine and
>bovine sperm. Mol Reprod Dev 35:189-96, 1993
>
>-Howard
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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