Detection of FITC oligos -Reply
Charles Kuszynski (CKUSZYNS@MAIL.UNMC.EDU)
Fri, 06 Jun 1997 09:29:21 -0500
It should be possible to remove the oligos on the outside of the cell with a
brief treatment with protease free DNAse. We have used a protocol like
this to examine the uptake of oligos by imaging. A second method could
include "pulse" labeling once you are sure the oligos are getting into the
cells.
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